Ratio Spectra First order Derivative Method has been developed for simultaneous estimation of Levosulpiride and Pantoprazole. The methanol and water was use as solvents. The method involved analytical signals were measured at maxima 258.2 nm and minima 251.1 nm for Levosulpiride and Pantoprazole in the first order derivative spectra (∆ λ = 8 nm and Scaling factor = 1) of the ratio spectra obtained by using 18 µg/ml PANTO and 75 µg/ml LEVO spectra as divisor for Levosulpiride and Pantoprazole respectively. Beer’s law obeyed in concentration range of 6-30 µg/ml for PANTO and 25-125 µg/ml for LEVO. The percent assay of marketed formulation was 99.79±1.1334 and 99.82±1.0182 of Levosulpiride and Pantoprazole respectively. The proposed methods are specific, accurate and precise so they are recommended for routine analysis.
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